Knockdown of SPAG6 inhibits the growth and dissemination of AML in vivo. (A) Kaplan-Meier curves showed the survival rate of mice injected with K562-shSPAG6 cells and HEL-shSPAG6 cells (n = 8 mice per group). (B-C) Representative examples of size and weight of spleens derived from leukemic mice with treatment as indicated. (D) White blood cell count in all the study groups. (E-F) Representative hematoxylin and eosin and immunohistochemical staining images of CD45 in BM, spleen, liver, and kidney, treated as indicated. Scale bar, 50 μm. **P < .01.