Stroke outcome at 24 hours is predicted by cortical perfusion measurements. C57BL/6 mice were subjected to the iCAT stroke model (60 minutes’ ischemia). (A) Representative flux image of the ipsilateral (L) and contralateral (R) cerebral cortex of a C57BL/6 mouse at 90 minutes’ post–stroke onset, in which the left inset depicts the area of ipsilateral hemisphere with severe hypoperfusion, defined as threshold <250 flux units, as quantified using moorReview 5.0 software. (B) Scatter plots represent the calculated area of severe hypoperfusion (as assessed in panel A) correlated with the overall degree of hypoperfusion within the same region, for each individual mouse. Data were analyzed by using XLSTAT (Addinsoft, Paris, France) to highlight any correlation between the level of hypoperfusion and stroke outcomes, where mice were grouped according to their outcome at 24 hours, including no infarct (no TTC-visible infarction) (i), large infarct (infarct visible on TTC > 16 mm³) (ii), and small infarct (infarct visible on TTC < 15 mm³) or mortality (iii), where an individual mouse was deceased before end point. (iv) Severe and moderate hypoperfusion were classified as LSCI flux <21% of baseline and LSCI flux between 21% and 37% of baseline, respectively. (C) Tabulated summary depicting area of hypoperfusion and correlated predictive outcome, based on the analysis presented in panel B. Experiments were conducted at ambient temperature <21°C (Site 1).