Schematic overview of the iCAT stroke model. (A) The iCAT model incorporates thrombotic occlusion of the CCA induced by electrolytic injury to allow for real-time monitoring of occlusion and recanalization events. (B) After thrombotic occlusion of the carotid artery, transient stenosis of the contralateral carotid artery induces ipsilateral cerebral hypoperfusion sufficient to induce infarction (<25% baseline flow), measurable with LDF over the MCA territory. Cerebral perfusion analysis with LSCI at 90 minutes’ post–stroke onset is predictive of 24-hour outcome (C), including behavioral deficit, infarct progression, and mortality (D). Elements of this image were created with BioRender.com and exported under a paid subscription. RBC, red blood cell.