Figure 6.
Mast cells and T cells are sources of IL-13 in MPN mouse models. (A) Uniform manifold approximation and projection (UMAP) of single-cell RNA sequencing analysis of BM cells from Jak2V617F recipient mice at 3 and 8 months after transplant (n = 2 per time point) colored by cell clusters. Data from all mice and time points are shown. (B) Uniform manifold approximation and projection of cells in prefibrotic (orange) and in fibrotic (green) stages. (C) IL-13 messenger RNA expression in sorted myeloid cells (CD11b+), T cells (CD3+), and mast cells (CD117+ FcεRIα+) from the BM of MPLW515L and Jak2V617F mice. (D) Mast cell tryptase staining in the BM of the indicated mice at early and late time points. (E) Mast cell tryptase staining of human BM biopsies from patients with MF stratified based on the fibrosis grade. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HPF, high powered field; MC, mast cells; Mk, megakaryocytic.

Mast cells and T cells are sources of IL-13 in MPN mouse models. (A) Uniform manifold approximation and projection (UMAP) of single-cell RNA sequencing analysis of BM cells from Jak2V617F recipient mice at 3 and 8 months after transplant (n = 2 per time point) colored by cell clusters. Data from all mice and time points are shown. (B) Uniform manifold approximation and projection of cells in prefibrotic (orange) and in fibrotic (green) stages. (C) IL-13 messenger RNA expression in sorted myeloid cells (CD11b+), T cells (CD3+), and mast cells (CD117+ FcεRIα+) from the BM of MPLW515L and Jak2V617F mice. (D) Mast cell tryptase staining in the BM of the indicated mice at early and late time points. (E) Mast cell tryptase staining of human BM biopsies from patients with MF stratified based on the fibrosis grade. GAPDH, glyceraldehyde-3-phosphate dehydrogenase; HPF, high powered field; MC, mast cells; Mk, megakaryocytic.

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