κ chain and variable gene use by PA, NA, and NB clones. (A) PA and NA clones used κ chains only. (B) Vκ1 was used preferentially by PA clones. (C) There was a striking preference for use of J_H6 by PA and NA clones relative to NB clones. (D) Percentage of clones using various heavy chain VDJ gene combinations. Data shown in B to D are based on sequences of 7 PA, 47 NA, and 40 NB clones. P values were calculated by Fisher exact test. ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, and ∗∗∗∗P < .0001. mAb, monoclonal antibody.