In vivo lineage-specific expression of MyeloVec. (A) Human HD CB CD34⁺ HSPCs were transduced and transplanted into sublethally irradiated NSG neonatal mice. Mice were harvested 18 weeks after transplant and stable BM VCN (B) and BM engraftment (C) were measured. Expression of each vector was evaluated across the different human hematopoietic lineages in the BM (D), PB (E), and spleen (F). (G) Kinetics of expression throughout neutrophil differentiation in the BM of engrafted mice. Data are presented as mean ± SD. Statistical significance was analyzed using a 2-way analysis of variance (ANOVA) followed by multiple paired comparisons for normally distributed data (Tukey test). All statistical tests were 2-tailed and P < .05 was deemed significant; ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001. Avg, average.