A combination of multimodal screening showed potential targets of EVI1 in AML cells. (A) Venn diagram of ChIP-seq data using anti-FLAG antibody, showing FLAG-EVI1 binding regions. Two 3× FLAG-tagged EVI1-AML samples and a sample from 32D-cl3 murine hematopoietic progenitor cells where FLAG-tag was knocked in to the 3′-end of the Mecom locus. (B) Database for Annotation, Visualization and Integrated Discovery (DAVID) analysis showing AML-specific EVI1-binding regions. (C) A model of RNA-seq experiments. Genes upregulated at both early and late points after EVI1 introduction were chosen for further analysis. (D) Venn diagram of genes with EVI1-binding (ChIP-seq), upregulation after EVI1 transduction (RNA-seq), and positive correlation between EVI1 expression (microarray). (E) The list of genes in the common population of Figure 2D. (F) A scheme of the screening assay using DsRed. (G) Relative enrichment of DsRed⁺ cells expressing shRNA against each gene through days 3 and 10, adjusted by the shLuciferase control. Significance was examined in comparison with the shLuciferase control. (H) qPCR showing the relative expression of Evi1, Erg, and Ccnd1 in GFP^high and GFP^low AML cells. Mean ± SD. ∗P < .05.