%VWF secretion from type 1 VWD and control ECFCs after basal and stimulated VWF release. Mean %VWF secretion from type 1 VWD and control ECFCs during basal VWF release and stimulated VWF release. VWF:Ag was measured using an ELISA. %VWF secretion was calculated using the following formula: VWF:Ag in media / (VWF:Ag in media + VWF:Ag in lysate)∗100. Data points indicate mean %VWF:Ag secretion for each cell line (performed in triplicate). Error bars represent the mean ±SD. Because of limited cell yield, this experiment could only be performed on 2/4 control-derived ECFCs and 6/8 type 1 VWD-derived ECFCs. Low cell yield also precluded statistical analysis.