Figure 2.
Confocal immunofluorescence microscopy of ECFCs after basal and stimulated VWF release. ECFCs derived from healthy controls (A) and patients with type 1 VWD (B). Images in the top row were taken during basal VWF release (PMA-) and images in the bottom row were taken after stimulated VWF release (PMA+). ECFC identification labels are displayed across the top of the figure. ECFCs were stained for VWF (green), VE-cadherin (red) and DAPI (blue). Images are shown at 63× magnification with white scale lines representing 40 μm. Images were taken using a Lecia TCS SP8 Confocal Microscope. (C) Pearson r correlation between VWF section measured by ELISA and WPB count quantified from confocal microscopy images of control and type 1 VWD ECFCs during basal and stimulated release. (D) The number of WPBs contained in control and type 1 VWD ECFCs after basal and stimulated secretion. ∗P<.05.

Confocal immunofluorescence microscopy of ECFCs after basal and stimulated VWF release. ECFCs derived from healthy controls (A) and patients with type 1 VWD (B). Images in the top row were taken during basal VWF release (PMA-) and images in the bottom row were taken after stimulated VWF release (PMA+). ECFC identification labels are displayed across the top of the figure. ECFCs were stained for VWF (green), VE-cadherin (red) and DAPI (blue). Images are shown at 63× magnification with white scale lines representing 40 μm. Images were taken using a Lecia TCS SP8 Confocal Microscope. (C) Pearson r correlation between VWF section measured by ELISA and WPB count quantified from confocal microscopy images of control and type 1 VWD ECFCs during basal and stimulated release. (D) The number of WPBs contained in control and type 1 VWD ECFCs after basal and stimulated secretion. ∗P<.05.

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