Storage of human RBCs with S1P promotes glycolysis and generation of ATP, at the expense of steady-state levels of metabolites from the PPP and other antioxidant pathways. (A) Human packed RBCs were stored in AS-3, either untreated or supplemented with 1, 5, or 10 μM of S1P. (B-C) Heat maps from metabolomics analyses of RBCs and supernatants from this experiment are shown in panels B and C, respectively. (D) Line plots (median ± ranges) for representative metabolites in glycolysis (pyruvate), ATP, PPP metabolites (ribose phosphate and pentose phosphate isobars, sedoheptulose phosphate), total glutathione (pools of reduced and oxidized), free fatty acids, and leukotrienes (LTA4 and LTB4). (E) A schematic representation of the results.