Self-renewal ability of pd-JAO-dc under hypoxia condition. (A) Propagating capacity of pd-JAO-dc after multiple passages in the pd-JAO system in normoxia and hypoxia conditions (N = 3 replicates). Mean and SEM are shown. ∗∗P ≤ .01 by 2-tailed unpaired t test. (B) Viability of pd-JAO-dc derived from serial dilution in 3D culture generated from P1 and P2 in the pd-JAO system (N = 3 replicates). Mean and SEM are shown. ∗P ≤ .05, ∗∗P ≤ .01 by 2-tailed paired t test.