Pluripotin potently inhibits the proliferation of Ba/F3 cells expressing FLT3^ITD, BCR-ABL, and Jak2^V617F. (A) The sigmoidal curve showing the viability of Ba/F3 cells expressing FLT3^ITD, BCR-ABL, and Jak2^V617F treated with dimethyl sulfoxide or increasing concentrations of pluripotin for 72 hours. IC₅₀ values for each cell line is indicated on the right. (B) Showing pFLT3, pERK1/2, and pSTAT5 levels determined by western blotting using total cells extracts incubated with different concentrations of pluripotin for 2 hours were probed with anti-pFLT3, anti-pSTAT5, anti-pERK1/2, anti-FLT3, anti-ERK1/2, and anti-STAT5 antibodies. (C) Scatter plot showing the extent of apoptosis in Ba/F3 cells expressing FLT3^ITD, BCR-ABL, and Jak2^V617F after the treatment with 100 nM of pluripotin. The quadrant in each plot show; Q1, early apoptotic cells (annexin V⁺); Q2, late apoptotic cells (annexin V⁺ and PI⁺); Q3, necrotic cells (PI⁺); and Q4, viable cells (annexin V⁻ and PI⁻). Representative cell proliferation data is shown from the mean of 2 independent experiments ± SD. Pluri, pluripotin.