Pluripotin induces apoptosis in leukemic cells while enriching the normal HSC progenitors. (A) Scatter plot showing the extent of apoptosis in Lin⁻ cells from the WT (left) and leukemic mice (right) on day 0 and after 6 days of drug treatment (vehicle, 0.1 mM and 1 mM pluripotin; 1 mM of gilteritinib). In each density plot quadrant show; Q1, Lin⁻ cells (Lin⁺ and annexin V⁻); Q2, apoptosis of differentiated cells (annexin V⁺ and Lin⁺); Q3, apoptosis in Lin⁻ cells (Lin⁻ and annexin V⁺); and Q4, Lin⁻ viable cells (annexin V⁻ and Lin⁻). (B) Bar plot showing total apoptotic cells from both Lin⁻ and Lin⁺ cells. (C) Bar plots showing the apoptotic cells from Lin⁻ cells. (D) Bar plot representing apoptotic cells from Lin⁺ cells. (E) Bar plots showing annexin V⁺ cells in CD34⁺ cells from the normal and AML donors before and after 6 days of drug treatment. (F) Bar plot depicting the colony-forming unit (CFU) numbers from the normal and AML CD34 cells treated with vehicle (dimethyl sulfoxide), pluripotin (0.1 mM and 1 mM) and gilteritinib (1 μM). All data presented are shown as mean ± SD collected from 2 independent experiments. ∗P < .05, ∗∗P < .01, and ∗∗∗P < .001.