MOF-driven thrombin generation is dependent on factors XII and XI. (A) Shown are representative TGA curves and quantitation of the lag time, peak thrombin, and endogenous thrombin potential when MOFs are added to normal human plasma alone or in combination with TF. Note that MOFs alone are capable of significant thrombin generation in the absence of TF. MOFs also augment TF-mediated thrombin generation when used in combination with either a low (1 pM) or high (5 pM) concentration of TF. (B) Shown are the results of TGA assays where MOFs were added to FXII-depleted human plasma (FXII^O) with or without TF. Note that depletion of FXII completely abrogated thrombin generation driven by MOFs alone. FXII elimination also abrogated the increase in thrombin generation seen when MOFs were added to a low concentration of TF. However, MOFs still significantly increased thrombin generation when added together with a higher concentration of TF. (C) Shown are the results of TGA assays where MOFs were added to FXI-depleted human plasma (FXI^O) with or without TF. FXI depletion also eliminated thrombin generation triggered by MOFs alone. Moreover, FXI depletion also completely mitigated the increase in thrombin generation seen when MOFs were added together with either low or high concentrations of TF. P values were generated using a Student t test; data represent the mean and SEM.