Iron parameters in MPN mouse models with mutations in JAK2 gene. (A) Blood cell parameters of different MPN mouse models. (B) Iron parameters and cytokine levels in serum. Note that logarithmic scales are used for iron regulatory cytokines (erythroferrone and hepcidin). (C) RNA sequencing–derived mRNA expression levels of genes involved in iron homeostasis in hematopoietic progenitors. All data are presented as mean ± standard error of the mean. Two-way ANOVAs with subsequent Tukey or Dunnett test were used for multiple-group comparisons. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001. MkPs, megakaryocyte progenitors.