Effects of parenteral iron injections on MPN phenotype. (A) Schematic drawing of the experimental setup for BM transplantations and iron injections. (B) Time course of body weight (n= 6-8 mice per group). (C) Complete blood counts of recipient mice (n = 6-8 mice per group) are shown at the indicated times. (D) Numbers of Mks per 10 HPFs counted in sternum sections of mice at original magnification ×400; n = 3 mice per genotype and treatment group. (E) Spleen weight at terminal analysis. (F) Iron parameters and regulatory cytokines at terminal analysis. Note that logarithmic scales are used for iron regulatory cytokines (erythroferrone and hepcidin). Group size was n = 4 to 8 mice per genotype and treatment group. Erythroferrone and hepcidin levels (shown on logarithmic scale) together with total iron binding capacity (TIBC) and transferrin saturation were determined in serum. All data are presented as mean ± standard error of the mean. Two-way ANOVAs with subsequent Dunnett posttest or Sidak posttest were used. One-way ANOVA with subsequent Tukey posttest was used to compare spleen weight (D). ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.