![Bone marrow–resident DCs express high levels of TLRs and inflammatory cytokines. (A) Representative flow plots showing gating strategy using Cx3cr1gfp/+ mice to sort DCs (MHCIIhigh CD11chigh CX3CR1-GFPhigh Gr-1– B220–), monocytes (CD115+ CX3CR1-GFPhigh B220–), and macrophages (MHCIIhigh CD169+ CX3CR1-GFP– Gr-1– B220–) in the bone marrow. (B) Principal component analysis (PCA) of RNA-sequencing data. (C) Expression of TLR receptors (fragments per kilobase [FPKM]). (D) Represented flow plots showing TLR1 or TLR2 cell surface expression (left panels: fluorescence minus one control [FMO]). Mean fluorescent intensity (ΔMFI) compared with FMO controls in the indicated cell population. (E) Basal expression of inflammatory cytokines/chemokines implicated in MDS pathogenesis that are expressed with an FPKM >2 in DCs, monocytes, or macrophages. Data represent the mean ± standard error of the mean. Statistical significance determined by using two-way analysis of variance (panels C and E) or one-way analysis of variance (panel D). ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/140/14/10.1182_blood.2022016084/4/blood_bld-2022-016084-gr1.jpeg?Expires=1743231994&Signature=u9nn2Go7O2May~LOetRaDoecmFh7wg-BMrdkXT4qSY794DjEs1DkA9EXYPXlA6zSmXVmR1VL9Jq7o6us3h8J5KlqEf3ONbrHqfzmIrpEKpMYcM-MTE1O4rZq8LfCEDFQIpRC5GZdPleVH1dVwuPu8CYHQrg7A6Xuhaf8Hcjj5JWpmHbbWXNQMGIWjZBxvL5bN-Emnc0yhx3BaOxJwGf~3crSobPCAVpd4Zo1VdsXugZztzahiv8BL-pvDLX4jUrlUjVS0zP6F0-PB3yY6HXgC~Bdrqti8U~tNOzpGtqUn7PHr-omsRe3IYjeHhTkWaFaErxpXnaq-MLJarXg1GB-EQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Bone marrow–resident DCs express high levels of TLRs and inflammatory cytokines. (A) Representative flow plots showing gating strategy using Cx3cr1gfp/+ mice to sort DCs (MHCIIhigh CD11chigh CX3CR1-GFPhigh Gr-1– B220–), monocytes (CD115+ CX3CR1-GFPhigh B220–), and macrophages (MHCIIhigh CD169+ CX3CR1-GFP– Gr-1– B220–) in the bone marrow. (B) Principal component analysis (PCA) of RNA-sequencing data. (C) Expression of TLR receptors (fragments per kilobase [FPKM]). (D) Represented flow plots showing TLR1 or TLR2 cell surface expression (left panels: fluorescence minus one control [FMO]). Mean fluorescent intensity (ΔMFI) compared with FMO controls in the indicated cell population. (E) Basal expression of inflammatory cytokines/chemokines implicated in MDS pathogenesis that are expressed with an FPKM >2 in DCs, monocytes, or macrophages. Data represent the mean ± standard error of the mean. Statistical significance determined by using two-way analysis of variance (panels C and E) or one-way analysis of variance (panel D). ∗P < .05, ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001.
