IL-1β expression in bone marrow DCs from healthy donors and patients with MDS. (A) Annotations of single-cell RNA-sequencing clusters from healthy donors and patients with MDS compared with reference data sets. (B) Expression of the indicated cDC2 marker genes. Bone marrow cells from 4 healthy donors were cultured overnight in PAM3CSK4 (10 ng/mL) or vehicle alone; DCs or monocytes were sorted by flow cytometry; and IL1B mRNA expression was quantified. (C) Representative flow plots showing gating strategy to identify human DC2-like cells. (D) IL1B mRNA expression data; significance determined by paired t-test. (E) Violin plots showing TLR1, TLR2, or IL1B mRNA expression in the indicated myeloid cell population; data are pooled single-cell RNA-sequencing data from 2 healthy donors (HD) and 4 patients with MDS. Significance determined by unpaired t-test. ∗P < .05, ∗∗∗P < .001, ∗∗∗∗P < .0001. ASDC, AXL+ DC; BaEoMa, basophil, eosinophil, mast cells; CLP, common lymphoid progenitor; Ctrl, control; EMP, erythrocyte megakaryocyte progenitor; ILC, innate lymphoid cell; NK, natural killer; pDC, precursor DC; pre-pDC, precursor plasmacytoid DC; Prog Mk, progenitor megakaryocytes; UMAP, Uniform Manifold Approximation and Projection.
