K3 deficiency results in anemia in mice. (A) Summary of experimental mouse strains. (B) Erythroid cells and macrophages were isolated from mouse BM using anti-Ter119 and anti-F4/80 beads, respectively, and K3 expression was determined on western blots probed with anti-K3 Ab, using anti-GAPDH as a loading control. Images are representative of 4 mice per strain. (C) Comparison of several hematologic parameters of mouse peripheral blood at baseline. (D) Comparison of RBC and Hb recovery in mice in response to PHZ-induced hemolytic anemia. (E) Comparison of RBC and Hb levels in mice during EPO-induced erythropoiesis. The data were acquired by Advia 120 Hematology System and are mean ± SD; mutant vs K3WT mice; ∗P < .05; n = 10 mice per group.