K3 deficiency impairs erythrocyte deformability and enhances osmotic fragility. (A,B) Osmotic gradient ektacytometry revealed increased Omin (A) and Ohyper (B) values in K3KO/EpoR-cre erythrocytes. Results are mean ± SD. ∗P < .05; n = 4; representative of 4 independent experiments. (C) Increased osmotic fragility of K3KO/EpoR-cre erythrocytes. Results are mean ± SD. ∗P < .05; n = 5; 3 independent experiments were performed. (D) Microfluidic assessment of erythrocyte deformability reveals an increased occlusion index. Results are expressed as mean ± SD. ∗P <.05; n = 5.