Summary of significant genes hit from etoposide CRISPR screen. (A) CRISPR/Cas9 loss-of-function screening overall schema. (B) Significant genes were identified by CRISPR screen at day 4 (early), day 12 (intermediate), and day 18 (late) in response to etoposide exposures, with EtoSGs highlighted in blue and EtoRGs highlighted in red (FDR < 0.1). (C) Volcano plots of gene hits with log₂(FC) values between the 3 exposures times of etoposide. (D) Rank plots of the top 10 EtoRGs and EtoSGs with their respective etoposide scores across 3 time points, with a higher negative score indicating increased etoposide resistance and a higher positive score indicating increased etoposide sensitivity. EtoRGs, etoposide resistance genes; EtoSGs, etoposide sensitive genes.