American Society of Hematology

Figure 1.

M-GFP-LV transduces LT-HSCs and HPCs in C57BL/6 mice. MND-GFP-LV (2.88 × 107 ifu per animal) was IO-injected in C57BL/6 mice (n = 4 per group). Untreated naive mice were used as control samples. (A) BM was isolated 1 week after LV delivery and stained for LT-HSCs (LSK-CD150+CD48−), HPC-1 (LSK-CD150−CD48+), HPC-2 (LSK-CD150+CD48+), and MPPs (LSK-CD150−CD48−). (B) GFP expression was measured in each subtype of LSK cells in untreated naive (top) and LV-treated (bottom) mice. From left to right, HPC-1, HPC-2, LT-HSC, and MPP. (C) Summary plot of GFP expression after subtracting the control samples in different subpopulations of LSK cells. The data are presented as means with standard deviation.

M-GFP-LV transduces LT-HSCs and HPCs in C57BL/6 mice. MND-GFP-LV (2.88 × 10⁷ ifu per animal) was IO-injected in C57BL/6 mice (n = 4 per group). Untreated naive mice were used as control samples. (A) BM was isolated 1 week after LV delivery and stained for LT-HSCs (LSK-CD150⁺CD48⁻), HPC-1 (LSK-CD150⁻CD48⁺), HPC-2 (LSK-CD150⁺CD48⁺), and MPPs (LSK-CD150⁻CD48⁻). (B) GFP expression was measured in each subtype of LSK cells in untreated naive (top) and LV-treated (bottom) mice. From left to right, HPC-1, HPC-2, LT-HSC, and MPP. (C) Summary plot of GFP expression after subtracting the control samples in different subpopulations of LSK cells. The data are presented as means with standard deviation.

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