G-GFP-LV transduction induces GFP expression in human megakaryocytes in vitro. (A) Human CD34⁺ cells isolated from G-CSF mobilized donors were cultured in SFEM II media supplemented with CC110 or MEG for 9 days. Preferential differentiation into megakaryocytes (CD41a⁺, CD42b⁺) was measured using flow cytometry. (B) Schematic of the self-inactivating LV genome encoding GFP under the platelet-specific promoter GpIbα is shown at the top. Human HSPCs were transduced with G-GFP-LV and cultured in MEG-supplemented SFEM media for 9 days before the expression was measured on flow. Top row of flow panels, untransduced cells; bottom row of flow panels, cells transduced at a multiplicity of infection (MOI) of 1. GFP expression was gated in CD41a⁻ cells (left panels) and CD41a⁺ cells (right panels).