G-GFP-LV transduction induces FVIII expression in human megakaryocytes in vitro and integrates polyclonally. Human CD34⁺ HSPCs were treated with G-F8-LVs (MOI = 5) and subsequently grown in MEG supplemented media for 14 days. (A) Intracellular FVIII expression was measured by flow cytometry. (B) MGS-PCR was performed on genomic DNA isolated from G-F8-LV transduced human HSPCs G-F8-LVfor RIS analysis. Clones contributing ≥1% of the detected pool are denoted by a colored bar. All remaining identified clones are grouped into the gray bar at the top of the plot. A total of 124 unique integration sites were found, none displaying clonal dominance. (C) Circle plot mapping RIS positions to the human genome (outer numbers indicate chromosome numbers). The heights of the histograms indicate the number of integrations found in a 1 Mbp bin.