Combined single-cell RNA and TCR sequencing defines expanded clones as TEMRA cells. (A) Sorted CD8⁺ T cells are clustered using their gene expression profile into naïve, TEMRA, central memory (CM), effector memory (EM), and CD161⁺ high cells. (B) Heatmap of genes in sorted CD8⁺ T cells differentially expressed in accordance with clusters, including naïve, TEMRA, CM, EM, and CD161⁺ high cells. (C) Large clones (top 10 clones) are predominantly within the TEMRA subset, shown in red. (D) Patients with a platelet count <30 × 10⁹/L have more TEMRA T cells compared with patients with a platelet count ≥30 × 10⁹/L.