Figure 6.
![HbS induces the production of proinflammatory cytokines in SCD mice. (A-B) Levels of TNF-α (A) and IL-6 (B) in the plasma of Townes HbAA and HbSS mice, 18 hours after IV injection with purified human HbS (2 μmol/kg, batches SLBV0862 [data represented as triangles] and SLCC7764 [data represented as dots]) or HbA (2 μmol/kg, batch SLCB7096), preceded or not by intraperitoneal injection with TAK-242 (1 mg/kg), 1 hour before (n = 3-11 mice per group). In each experiment, the same batch of HbS was injected into HbSS and HbAA mice. ∗∗∗P < .001; ∗∗P < .01; ∗P < .05 by Mann-Whitney test.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/140/18/10.1182_blood.2021014894/6/blood_bld-2021-014894-gr6.jpeg?Expires=1735807398&Signature=Xf2hNnwjju-5vvxfCJlzLUEV-eSFWna3jJ4lg5gWtc-94nAoNCvO-G74VTVd8seXPXXKG~9hWk12q~kwY~T8~2oxMBJPBNCeFIydxSLZOJ10IWVc3UuM9iCI033kzvvWDaNWXE97o6PqPAxFEG-yLbpzIGCa95lbtgdmDi7mXlFu3w88b644LbA9oePPxGcRYwbUtQvO6sojeutWIpuXOoqN~mOtLEuBAPzQ6mB33mTeVE9~w3TkP5Jd9HDQvcYt~9pXuxyZvCfZedIk5pvWmHuxY3Gpxd1WkVN4myi9gXGGp4R60w9W3Jd4c5WVJaJCLmF-0Zmzt4ZzqlX5tJbY~Q__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
HbS induces the production of proinflammatory cytokines in SCD mice. (A-B) Levels of TNF-α (A) and IL-6 (B) in the plasma of Townes HbAA and HbSS mice, 18 hours after IV injection with purified human HbS (2 μmol/kg, batches SLBV0862 [data represented as triangles] and SLCC7764 [data represented as dots]) or HbA (2 μmol/kg, batch SLCB7096), preceded or not by intraperitoneal injection with TAK-242 (1 mg/kg), 1 hour before (n = 3-11 mice per group). In each experiment, the same batch of HbS was injected into HbSS and HbAA mice. ∗∗∗P < .001; ∗∗P < .01; ∗P < .05 by Mann-Whitney test.
