Figure 1.
![Overview of the role of genome/gene-editing platforms. Genome-editing nucleases (TALEN, ZFN, and CRISPR-Cas9) induce DSBs within the nuclear DNA at specific sites, which can subsequently be repaired using nonhomologous end-joining (NHEJ) or homology-directed repair (HDR) pathways that assist in targeted modification of the DNA. In contrast, gene-editing RNA interference strategies (using shRNA or small interfering RNA [siRNA]) bind to complementary messenger RNA sequences within the cytoplasm and cause its degradation. mRNA, messenger RNA; RISC, RNA-induced silencing complex. Created with www.BioRender.com.](https://ash.silverchair-cdn.com/ash/content_public/journal/bloodadvances/7/10/10.1182_bloodadvances.2022008966/1/blooda_adv-2022-008966-gr1.jpeg?Expires=1735812092&Signature=oUWM6vRs7u-K2O9S~ZMviTqrYdvxvYzU6SKBJ5msSyCQcLZPgO7~yRz6NUQTL2DvtaMGEYfFQwI0PmHcNSY~HHnIrfKztDrEkzk-vYRCuCLVkQ-OwMYrW4DsM80pI~liYAh~pMKPEm2EXrLKC23DbQY-wfWvE--75gZocGtinIN7DKm0WaYIGMsDQiFJemFL-NtxyIZBQyexEgeC9ztgqR~A1sw7eYhZqET4SFEgmbyGfnpru4Fh6VZCxZXlN7zfIy-rjaGvuaaAEQVOotzGjXJGJlnXGU0MCDnNgT4xkhsaVrZWUxTO6iLtBQdI6~09mVytSXVDVMpbm0l7TEyFlw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Overview of the role of genome/gene-editing platforms. Genome-editing nucleases (TALEN, ZFN, and CRISPR-Cas9) induce DSBs within the nuclear DNA at specific sites, which can subsequently be repaired using nonhomologous end-joining (NHEJ) or homology-directed repair (HDR) pathways that assist in targeted modification of the DNA. In contrast, gene-editing RNA interference strategies (using shRNA or small interfering RNA [siRNA]) bind to complementary messenger RNA sequences within the cytoplasm and cause its degradation. mRNA, messenger RNA; RISC, RNA-induced silencing complex. Created with www.BioRender.com.
