Transcriptional differences between HLEC and vascular endothelial cells. (A) PCA of bulk RNA-seq results. CV HLEC and periportal HLEC were defined according to CIBERSORTx results. (B) Uniform Manifold Approximation and Projection (UMAP) showing the projection of single cell RNA-seq of arterial endothelial cells onto human liver single cell RNA-seq data. Note that most of the arterial endothelium projects to periportal HLECs and none projects to the CV HLEC cluster. (C) Volcano plot presenting the transcriptional differences between CV HLEC and HUVEC (CV HLEC upregulated genes on the right). (D) Gene set enrichment analysis revealing the HALLMARK enriched pathways in CV HLEC and HUVEC at FDR <0.05.