Expression of coagulation factors in HLEC vs HUVEC and prioritized transcription factor drivers. (A) Bulk RNA-seq (upper panel) and reverse transcriptase quantitative polymerase chain reaction based (lower panel) expression of coagulation genes (F8, F10, PROS1, SERPINC1, VWF) in CV HLEC and HUVEC (results presented as mean +/− standard error of the mean). (B) Enzyme-linked immunosorbent assay of 24 hour supernatants of HUVEC and FCGR2B+ HLEC for PROS1, FX, VWF, and activity assay for FVIII. (C) Prioritized denovo motifs enriched in genes downregulated (left) or upregulated (right) in CV HLEC compared with HUVEC (top panel). Corresponding enrichment of known E2F and RXR:RAR motifs in CV HLEC and HUVEC (middle panel), box plot with the expression of E2F3, E2F8 and RARA in CV HLEC and HUVEC in bulk RNA-seq (lower panel). (D) Bulk RNA-seq (upper panel) and reverse transcriptase quantitative polymerase chain reaction based (lower panel) expression of E2F pathway genes (E2F3, E2F8, CCNB2, CDK1, XPO1) in CV HLEC and HUVEC (results presented as mean +/− standard error of the mean).