Doxorubicin-induced activation of coagulation in a tissue factor- dependent manner. Levels of markers of activation of coagulation (A) Prothrombin fragment 1+2 and (B) TAT complexes were measured in the plasma of wild-type mice (n = 4-5/group) 24 hours after administration of doxorubicin (Dox) or vehicle control (Veh). Levels of procoagulants, (C) extracellular vesicle tissue factor (EV-TF), and (D) cell-free DNA (cfDNA) were measured in the plasma of wild-type mice (n = 4-5/group) 24 hours after administration of doxorubicin or vehicle control. Levels of TAT were measured in the plasma of (E) TF^low and TF^wt or (F) F12^+/+ and F12^-/- mice (n = 6-8/group) 24 hours after administration of doxorubicin (20 mg/kg). ∗P < .05 Mann-Whitney tests. Data are represented as individual values with the median and interquartile ranges.