Transplantation with SA-FasL–engineered splenocytes results in reduced levels of anti-inflammatory and increased levels of regulatory cytokines in GVHD target tissues. Total RNA was isolated on day 7 (A) and 21 (B) from the liver, small intestine, and large intestine of F1 recipients of C57BL/6 bone marrow cells (BM) and BM cells cotransplanted with SA-FasL–engineered (BM + SA-FasL-spleen) or nonengineered (BM + spleen) splenocytes. The transcripts for the indicated cytokines and chemokines were analyzed using TaqMan RT-qPCR. Fold change expression (2^–ΔΔCt) was calculated with respect to GAPDH as a house-keeping gene and BM-only recipients. Data are representative of 2 independent experiments and shown as mean ± SEM. For comparisons, 1-way ANOVA with Tukey post hoc test was used in panels A-C. ANOVA, analysis of variance; SEM, standard error mean. ∗P < .05; ∗∗P < .01; ∗∗∗P < .001; ∗∗∗∗P < .0001.