Figure 1.
Characterization of HOD × GFP RBCs. (A) Schematic of mouse breeding used to produce HOD × GFP mice. (B-C) Flow cytometric analysis displayed as dot plots (B) or histograms (C) of WT RBCs, HOD RBCs, GFP RBCs, and HOD × GFP RBCs after staining with anti-Duffy antibody. (D) Flow cytometric analysis of WT RBCs, HOD RBCs, GFP RBCs, and HOD × GFP RBCs after staining with anti-HEL antibody. (E) Detection of endogenous GFP expression by flow cytometric analysis of WT RBCs, HOD RBCs, GFP RBCs, and HOD × GFP RBCs. (F) Representative images of HOD × GFP RBCs. (G) Flow cytometric analysis of WT (B6) RBCs, GFP RBCs, HOD × GFP RBCs, and WT RBCs with chemically coupled recombinant GFP (positive control) after staining with anti-GFP antibody. All panels show representative data from experiments reproduced 2 times.

Characterization of HOD × GFP RBCs. (A) Schematic of mouse breeding used to produce HOD × GFP mice. (B-C) Flow cytometric analysis displayed as dot plots (B) or histograms (C) of WT RBCs, HOD RBCs, GFP RBCs, and HOD × GFP RBCs after staining with anti-Duffy antibody. (D) Flow cytometric analysis of WT RBCs, HOD RBCs, GFP RBCs, and HOD × GFP RBCs after staining with anti-HEL antibody. (E) Detection of endogenous GFP expression by flow cytometric analysis of WT RBCs, HOD RBCs, GFP RBCs, and HOD × GFP RBCs. (F) Representative images of HOD × GFP RBCs. (G) Flow cytometric analysis of WT (B6) RBCs, GFP RBCs, HOD × GFP RBCs, and WT RBCs with chemically coupled recombinant GFP (positive control) after staining with anti-GFP antibody. All panels show representative data from experiments reproduced 2 times.

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