Treg phenotype and STAT5 activation. (A) FoxP3 mean fluorescence intensity on Tregs from HDs and patients with DOCK11. Treg are defined by CD4⁺ CD25⁺ CD127_low as depicted in the graph on the right side of the panel. (B) Representative dot plots of CD3⁺ CD4⁺ Foxp3⁺ Helios⁺ cells from HDs and 2 patients with DOCK11. (C) Representative histograms of phospho-STAT5 in T cells, CD25⁺ T cells, and B cells from HDs and patients with DOCK11. Cells were stimulated for 60 minutes with IL-2. Results for unstimulated (NS) cells are shown with the black peak. (D) Kinetics of phospho-STAT5 expression upon IL-2 stimulation: unstimulated (NS), 15 and 60 minutes. Mean fluorescence intensity (MFI) of phospho-STAT5 in T cells, CD25⁺ T cells, MAIT cells, and B cells from HDs and patients with DOCK11.