BMI1 occupies proximal CpG islands at the IGF2BP1, IGF2BP3, and LIN28B genes. (A) Heatmaps of BMI1, H2AK119ub1, and H3K27me3 peaks identified from CUT&RUN experiment in control or BMI1-depleted HUDEP2 cells. (B-C) Scatter plots of H2AK119ub1 (B) and H3K27me3 (C) enrichments in control and BMI1-depleted HUDEP2 cells. Differential analysis was performed using DESeq2 and DiffBind packages. Differential peaks were identified with FDR < 0.05 and are labeled in red (n = 2 biological replicates). (D) Chromatin occupancy of BMI1 and enrichment of H2AK119ub1 and H3K27me3 at the IGF2BP1 and LIN28B genes in control and BMI1-depleted HUDEP2 cells. Chromatin occupancy of BMI1 in primary adult erythroblasts was used for comparison. CpG island track (hg38) was obtained from the University of California, Santa Cruz (UCSC) genome browser. (E) Enrichment of H3K27me3 and H3K27ac at the IGF2BP1 and LIN28B genes in primary erythroblasts derived from CD34⁺ HSPCs isolated from fetal liver (fetal), cord blood (newborn), and peripheral blood (adult); n = 2 healthy donors.