Figure 3.
ETS1 drives cell growth in NA-ATLL. (A) Genes were ranked from most upregulated to most downregulated in NA-ATLL compared with J-ATLL cell lines. ETS1 target genes were significantly enriched in genes upregulated in NA-ATLL cell lines. (B) IPA pathway analysis of ETS1 target genes upregulated in NA-ATLL cell lines compared with J-ATLL cell lines and NA-ATLL primary tumor cells compared with normal PBMC and CD4 cells. (C-E) NA-ATLL cell lines ATL18, ATL21, and ATL29 were transfected with siRNA targeting ETS1 or nontarget control. Equal number of viable cells were plated 24 hours after transfection, and relative cell number was determined by Cell Titer Blue assay at 48 hours (ATL18 and ATL21) or 72 hours (ATL29) after transfection. Two-sided t test, mean ± SE. Data shown are representative of 2 to 3 independent transfection experiments per cell line. Knockdown of ETS1 gene expression was assessed by qPCR (supplemental Figure 10).

ETS1 drives cell growth in NA-ATLL. (A) Genes were ranked from most upregulated to most downregulated in NA-ATLL compared with J-ATLL cell lines. ETS1 target genes were significantly enriched in genes upregulated in NA-ATLL cell lines. (B) IPA pathway analysis of ETS1 target genes upregulated in NA-ATLL cell lines compared with J-ATLL cell lines and NA-ATLL primary tumor cells compared with normal PBMC and CD4 cells. (C-E) NA-ATLL cell lines ATL18, ATL21, and ATL29 were transfected with siRNA targeting ETS1 or nontarget control. Equal number of viable cells were plated 24 hours after transfection, and relative cell number was determined by Cell Titer Blue assay at 48 hours (ATL18 and ATL21) or 72 hours (ATL29) after transfection. Two-sided t test, mean ± SE. Data shown are representative of 2 to 3 independent transfection experiments per cell line. Knockdown of ETS1 gene expression was assessed by qPCR (supplemental Figure 10).

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