Capacity of miR-146b-5p concentrations of predicting TTFT in CLL. (A) Association between miR-146b-5p concentrations and IGHV mutational status in 224 CLL cases. The numbers of CLL cases (subdivided into different quartiles according to miR-146b-5p concentrations) are indicated in each bar. (B) Kaplan-Meier curves of the 6-year TTFT probability of cases stratified according to miR-146b-5p expression. Cases with the lowest miR-146b-5p (first quartile) expression had a 47% probability of prolonged TTFT compared with that of 73%, 83%, and 79% of cases within the second, third, and fourth quartile, respectively. Statistical significance of associations between individual variables and survival was calculated using the log-rank test. (C) Kaplan-Meier curves showing TTFT probability of cases stratified in different quartiles defined by miR-146a-5p expression. Statistical significance of associations between individual variables and survival was calculated using the log-rank test. (D) Kaplan-Meier curves comparing TTFT of miR-146b-5p–low (first quartile) or miR-146b-5p higher expression (second to fourth quartiles) in IGHV-UM cases and in (E) IGHV-M cases. Statistical significance of associations between individual variables and survival was calculated using the log-rank test. (F) Cox multivariate analysis of IGVH-UM CLL cases (n = 80) showing that low miR-146b-5p expression (first quartile) maintains an independent prognostic impact in the presence of other prognostic indicators (P = .035).