Biochemical, immunological, and pharmacological parameters. (A) List of deviated laboratory data. Arrows indicate the values of analytes which were ±20% or more of the corrected preload value. ^§Preload values were corrected using a correction coefficient (supplemental Table 3; supplemental Methods). ALT, alanine aminotransferase; aPTT, activated partial thromboplastin time; AST, aspartate aminotransferase; CPK, creatine phosphokinase; LDH, lactate dehydrogenase; n.t., not tested. ∗Only approximately 10 mL was infused because of the manifestation of rash with wheal. (B) C-reactive protein (CRP) and complement system parameters of the subjects in cohort 3. Slightly increased CRP was found in 3 subjects regardless of the infusion volume of HbV suspension. No significant change in complement system parameters was found. CH50, 50% hemolytic complement activity. (C) Plasma Hb concentration profile of subjects in cohort 3 (100-mL dosing). In cohort 3, 2 subjects completed a 100-mL infusion of HbV suspension: (a) sequential change of plasma color and transparency; (b) plasma Hb concentrations; and (c) pharmacokinetic parameters. AUC∞, area under the plasma concentration – time curve from time zero until infinity; AUC_t, area under the plasma concentration – time curve from time zero until t; CL/F, apparent total body clearance; C_max, maximum plasma concentration; MRT_t, mean residence time from time zero until t; Kel, elimination rate constant; T_1/2, elimination half-life; T_max, time of maximum plasma concentration.