Single administration of hIL-7-hyFc has no significant effect on Treg cell expansion. (A) Gating strategy for Treg cells, frequency of Treg cells among CD4⁺ T cells, and absolute counts of Tregs. Each value of cohorts and placebo are shown as mean (± standard error of the mean) at the indicated time points. (B) Upper plots display the difference in Ki-67 expression among Tregs (Foxp3⁺CD127^−/loCD25⁺ CD4⁺; red) and Foxp3⁻CD4⁺ (blue) T cells. Bottom plots display the difference in Ki-67 expression among Treg cells (red), CD4⁺ T cells (blue), and CD8⁺ T cells (green). For each cohort, the frequency of Ki-67⁺ cells in each T-cell subset is shown. P values were calculated with a Friedman one-way ANOVA test. ∗∗P < .01, ∗P < .05; ns, not significant.