Figure 6.
Structure function analysis of RBM12 RRM domains. Two independent RBM12+/− clones were infected with lentivirus containing conditional RBM12 overexpression (OE) and luciferase control constructs. (B and D) Plotted are means ± standard deviation. (A) Left: Schematic of various forms of RBM12 OE constructs. Right: Representative Western blot of mutant RBM12 OE constructs with indicated antibodies. (B) HbF flow cytometry (left) and qRT-PCR of HBG1/2 mRNA as percentage of total β-like globin transcripts (right) at day 5 of differentiation. (C) Top: Schematic of RBM12 truncated OE constructs. Bottom: Representative Western blot of RBM12 truncated OE constructs with indicated antibodies. (D) HbF flow cytometry (left) and qRT-PCR of HBG1/2 mRNA as percentage of total β-like globin transcripts (right) at day 5 of differentiation.

Structure function analysis of RBM12 RRM domains. Two independent RBM12+/− clones were infected with lentivirus containing conditional RBM12 overexpression (OE) and luciferase control constructs. (B and D) Plotted are means ± standard deviation. (A) Left: Schematic of various forms of RBM12 OE constructs. Right: Representative Western blot of mutant RBM12 OE constructs with indicated antibodies. (B) HbF flow cytometry (left) and qRT-PCR of HBG1/2 mRNA as percentage of total β-like globin transcripts (right) at day 5 of differentiation. (C) Top: Schematic of RBM12 truncated OE constructs. Bottom: Representative Western blot of RBM12 truncated OE constructs with indicated antibodies. (D) HbF flow cytometry (left) and qRT-PCR of HBG1/2 mRNA as percentage of total β-like globin transcripts (right) at day 5 of differentiation.

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