Lu/BCAM clusters are present on malaria altered uRBCs. (A) Surface expression of Lu/BCAM on control RBCs (control) and malaria culture–derived uRBCs (MC). Data represent the mean fluorescence intensities (MFI) of control RBCs and MC uRBCs. n = 6. (B) Percentage of Lu/BCAM⁺ RBCs in control RBCs and MC-derived uRBCs. n = 6. (C) Surface expression of Lu/BCAM on control RBCs and RBCs exposed to CM. The data represent the MFI values of control RBCs and CM RBCs. n = 7. (D) Percentage of Lu/BCAM⁺ RBCs in control RBCs and CM-exposed RBCs. n = 6. (E) Imagestream analysis was performed to visualize surface expression of Lu/BCAM (green) on control RBCs, uRBCs derived from malaria culture (MC uRBCs), and RBCs exposed to CM (magnification ×60). (F) MFIs of Fluo-4–stained uRBCs from MC (y-axis) were plotted against the surface expression of Lu/BCAM (x-axis). n = 16. (G) Surface expression of CD44 on control RBCs (control) and MC-derived uRBCs. Data represent the MFIs of control RBCs and MC uRBCs. n = 5. (H) Percentage of CD44⁺ RBCs in control RBCs and MC-derived uRBCs. n = 5. (I) Surface expression of CD44 on control RBCs and CM RBCs. Data represent the MFIs of control RBCs and CM RBCs. n = 8. (J) Percentage of CD44⁺ RBCs in control RBCs and CM-exposed RBCs. n = 8. (K) Representative images of CD44 (green) stained control RBCs, uRBCs from malaria culture (MC uRBCs) and RBCs exposed to malaria CM visualized by imaging flow cytometry (magnification ×60). Wilcoxon matched-pairs signed-rank test.