Flowchart of MCB establishment from the patient’s peripheral blood. From the patient’s peripheral blood, mononuclear cells were purified and subjected to reprogramming to iPSCs using SNL cells as feeder cells (SNL-iPSC) and then transferred on Matrigel (M-iPSC). Using the revised "PSC-sac" method, M-iPSCs were differentiated into hematopoietic progenitor cells. Then, under megakaryocyte differentiating condition, c-MYC, BMI-1, and BCL-XL were sequentially transduced by lentiviral vectors to establish imMKCLs. A single imMKCL clone was selected based on expandability and platelet production and stocked as seed cell banks (SCB) and then as an MCB in liquid nitrogen.