Figure 4.
In vitro functional assessment of iPSC-PLTs shows the comparability with blood donor-derived platelets. (A) Representative flow cytometry images of P-selectin expression and PAC-1 binding of iPSC-PLTs (batch 09 and 17) with or without 100 μM ADP and 40 μM TRAP-6. (B) Flow cytometry data as in panel A for 4 batches of iPSC-PLT samples before processing, after filtration, and after the second ACP215 centrifugation for washing and after irradiation. (C) Aggregation assay. iPSC-PLTs (batch 07 and 09) were stimulated with 50 μM ADP or 5, 10, and 20 μg/mL collagen. (D) Autologous iPSC-PLTs of batch 16 in a blood bag were stored in a platelet preservation shaker. At the manufacturing date and after storage for 1, 2, 4, 6, and 8 days, the samples were tested for platelet count, CD42b expression, annexin V binding and in vitro function, and PAC-1 binding and P-selectin expression with or without ADP + TRAP-6 stimulation.

In vitro functional assessment of iPSC-PLTs shows the comparability with blood donor-derived platelets. (A) Representative flow cytometry images of P-selectin expression and PAC-1 binding of iPSC-PLTs (batch 09 and 17) with or without 100 μM ADP and 40 μM TRAP-6. (B) Flow cytometry data as in panel A for 4 batches of iPSC-PLT samples before processing, after filtration, and after the second ACP215 centrifugation for washing and after irradiation. (C) Aggregation assay. iPSC-PLTs (batch 07 and 09) were stimulated with 50 μM ADP or 5, 10, and 20 μg/mL collagen. (D) Autologous iPSC-PLTs of batch 16 in a blood bag were stored in a platelet preservation shaker. At the manufacturing date and after storage for 1, 2, 4, 6, and 8 days, the samples were tested for platelet count, CD42b expression, annexin V binding and in vitro function, and PAC-1 binding and P-selectin expression with or without ADP + TRAP-6 stimulation.

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