In vivo functional assessment of iPSC-PLTs. (A) In vivo circulation in thrombocytopenic rabbit models for 3 batches of iPSC-PLTs as measured by the percentage of human and rabbit platelets in peripheral blood using flow cytometry. (B) In vivo hemostasis in thrombocytopenic rabbit models for iPSC-PLTs, as measured by the bleeding time of the ear incision before and after the transfusion of human iPSC-PLTs (batch 09). The maximum time was set to 600 seconds. (C) Circulation of iPSC-PLTs in NOG mice by IVIS imaging: imMKCLs or iPSC-PLTs expressing Venus-Akaluc were injected into NOG mice. Phosphate-buffered saline was injected for the control group. After 2, 6, 48, 72, and 168 hours, the mice were subjected to IVIS imaging.