ΔdblGATA mice have defective priming in EAE. WT and ΔdblGATA mice were immunized for EAE induction and euthanized on day 8 after immunization (n = 4-5 per group; 2 independent experiments). (A-C) Analysis of inguinal dLN cells are shown in panels A-C. (A) Total number of CD45⁺ cells per mouse in inguinal dLNs. (B) Number of CD45⁺CD11b⁺, CD45⁺CD11b⁺CD11c⁺, CD11b⁺Ly6C^hiLy6G^lo/−, and CD4⁺ cells. (C) Cells from dLNs were stimulated with 20 μg/mL PLP_180-199, and proliferation of cells was measured by [³H]adenine incorporation. (D-E) Analysis of splenocytes. (D) Total number of cells in the spleens of mice with EAE. (E) [³H]adenine incorporation assay for PLP_180-199–stimulated splenocytes showing the average proliferation per mouse. (F-K) Analysis of the CNS. (F) Number of CD45^hi cells. (G) Number of CD45^hiCD11b⁺, CD45^hiCD11b⁺CD11c⁺, CD11b⁺Ly6C^hiLy6G^lo/−, and CD4⁺ cells. (H) Representative flow cytometry plots showing MHC-II expression in myeloid DCs present in the CNS of the EAE mice. Gated on CD45^hiCD11c⁺CD11b⁺ cells. (I) Quantification of the frequency of MHC-II^hi and MHC-II^lo cells among CD45^hiCD11b⁺CD11c⁺ cells. (J) Histograms normalized to the mode of data depicting MHC-II, CD80, and CD86 expression among CD45^hiCD11b⁺CD11c⁺ cells. (K) Quantification of the median fluorescent intensity of MHC-II, CD80, and CD86 expression among CD45^hiCD11b⁺CD11c⁺MHC-II⁺ cells. Error bars are SEM, and significance was calculated using unpaired Student t test.