ΔdblGATA mice have reduced numbers of HSCs and myeloid cell progenitors. (A) Flow cytometry plots showing frequencies of CD11b⁺ and CD11c⁺ cells among CD45⁺ PBMCs in naïve WT and ΔdblGATA mice. (B) Frequencies of CD11b⁺, CD11c⁺, CD19⁺, CD4⁺, and CD8⁺ cells among CD45⁺ PBMCs. N = 4 to 13 per group. (C) Frequencies of Ly6C^hiLy6G^lo/− monocytes among CD11b⁺ PBMCs. N = 19 to 22 per group. (D-I) Quantification of hematopoietic progenitors in the BM of WT and ΔdblGATA mice. N = 5 to 8 per group, compiled from 2 independent experiments. (D) Flow cytometry plots depicting the expression of CD117 and CD34 among lineage negative cells (Lin⁻; CD3⁻B220⁻CD11b⁻TER119⁻Gr-1⁻). (E) Frequencies of CD117⁺Sca1⁻ cells among Lin⁻ cells. (F) Frequencies of MP populations among Lin⁻ cells. Full gating and population definitions can be found in the supplemental Figure 3. (G) Frequencies of CD117⁺Sca1⁺ cells among Lin⁻ cells. (H) Frequencies of long-term HSCs (LT-HSCs) among Lin⁻CD117⁺Sca1⁺ (KSL) cells. (I) Number of LT-HSCs and short-term HSCs/multipotent progenitors per mouse. Significance was determined by a 2-tailed unpaired t test. Error bars are SEM. CMP, common myeloid progenitor; GMP, granulocyte-myeloid progenitor; GP, granulocyte progenitor; MDP, monocyte-dendritic cell progenitor; cMoP, common monocyte progenitor.