Cultured spread MKs from mutant mice exhibit reduced PLS formation, F-actin content, and β1-integrin activation. (A) Representative confocal images of cultured BM-derived MKs from control (Adapfl/fl), ADAP-, PFN1-, WASp-, and ARP2/3-deficient mice spread on Horm collagen and stained for F-actin (red), the active form of β1-integrin (cyan), and the nucleus (blue). Scale bars represent 50 μm (left) and 20 μm (right). (B) Mean fluorescence intensity of active β1-integrin, (C) F-actin, and (D) density of PLS in the lowest optical section of spread MKs on Horm collagen, normalized to their respective control cells. Controls are indicated by a dashed line (B-D). (B-D) 35 to 59 MKs per genotype were analyzed. The data points represent individual MKs.
