Loss of α4A- and β1-tubulins severely impairs hemostatic and thrombotic responses in vivo. (A) Percentage of bleeders over time following a 3-mm tail-tip amputation for WT, KOA4A (A4A), KOB1 (B1), and DKO mice and romiplostim-treated DKO mice (DKO+R). Results are represented as Kaplan-Meier survival curves. For romiplostim-treated animals, mice were administered a single dose (100 μg/kg) 6 days prior to the experiment. N > 6 per group. Statistical analysis was conducted by the log-rank test. (B) Representative fluorescence micrographs of thrombi formed at 14, 24, and 40 minutes after topical application of FeCl₃ to the carotid artery of WT, KOA4A (A4A), KOB1 (B1), and DKO mice. Platelets were labeled by 3,3'-dihexyloxacarbocyanine iodide injection. (C) Thrombus area quantification after topical application of FeCl₃ to the carotid artery of WT, KOA4A (A4A), KOB1 (B1), and DKO mice. Results are expressed in mm² as a function of time (minutes). N > 5 per strain. (D) Representative scanning electron micrographs of WT and DKO thrombi in transsectioned carotid arteries that have been fixed 7 minutes after topical application of FeCl₃. (E) Percentage of survival over time of WT, KOA4A (A4A), KOB1 (B1), and DKO mice in a venous thromboembolism model following an injection in the jugular vein of a mixture of collagen (0.8 mg/kg) and adrenaline (60 μg/kg). Results are represented as Kaplan-Meier survival curves. N > 3 per group. Statistical analysis was conducted by the log-rank test. Scale bars, 300 μm (B) and 2 μm (D).