NOTCH1 agonism alters cytokine production of CAR-T derived from CD4⁺ T_N. (A) Luminex quantification of cytokines in culture medium after 24 hours of coculture of N1 and control CD4⁺ CAR-T with irradiated CD19⁺ Raji or K562 tumor cells. Dotted lines indicate limits of detection. N = 8 donors. Ratio-paired 2-tailed Student t test. ∗P < .05; ∗∗P < .01; ∗∗∗P < .005; ∗∗∗∗P < .001. (B) Intracellular staining for IL-22 and IFNγ after restimulation of N1 and control CD4⁺ CAR-T for 5 to 8 hours with PMA/ionomycin or CD19⁺ Raji tumor. Left: plots depict 1 donor representative of 7. Right: quantification of IL-22⁺ cell frequencies. Ratio-paired 2-tailed Student t test. ∗∗∗∗P < .001. n.s., not significant.