Mnk1 regulates cPLA2 activity and thromboxane production by platelets. (A-B) Total (A) and phosphorylated (B) cPLA2 protein expression was assessed by immunoblot in unstimulated or 2MeSADP-stimulated (10 nM, 5 minutes) platelets from WT and Mnk1 KO mice. β-Actin was used as a loading control. Immunoblots are representative of n = 3 independent experiments. (C-D) Washed platelets from WT and Mnk1 KO mice were left unstimulated (US) or stimulated with collagen (2 μg/ml), 2MeSADP (2 nM), or AYPGKF (150 μM) 10 minutes. Then, platelet cPLA2 activity (C) and TXB2 production (D) were assessed (n = 3-5 mice per group, ∗P < .05). M, marker.