In vivo tumor control by iDuo NK cells. (A) Schematic for experiments assessing iDuo NK cell antitumor function as a single agent in vivo. NSG mice were injected IV with 1 × 105 Nalm6 cells expressing firefly luciferase. After allowing the tumors to establish for 1 day, mice either received no treatment (n = 8) or IV injections of 1 × 107 iDuo NK cells (n = 10) along with 5 × 104 U IL-2 on days 1, 7, and 14. (B) Bioluminescence images throughout the first 3 weeks of the experiment. (C) Graphical representation of cumulative BLI data for the entire 7 weeks of the experiment. Statistical significance was determined using a two-way ANOVA. Shown is mean ± standard deviation. (D) Kaplan–Meier curves for the Nalm6 adoptive transfer experiment. Results are from two independent experiments. Statistical significance was determined using a log-rank (Mantel–Cox) test. ∗∗∗∗P ≤ .0001.
